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Plant Tissue Culture,

Edition 4 Techniques and Experiments

Editors: By Sunghun Park

Publication Date: 19 Feb 2021

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Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.

Key Features

Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement
Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
Contains the latest advances and updates since the previous edition published in 2012

About the author

By Sunghun Park, Professor, Department of Horticulture and Natural Resources, Kansas State University

1. History of plant tissue culture2. Setup of a tissue culture laboratory3. Media components and preparation4. Explant preparation5. Contamination6. Callus induction7. Regeneration and morphogenesis8. Woody shrubs and trees9. Haploid plants from another culture10. Embryo rescue11. Meristem culture for virus-free plants12. In Vitro propagation for commercial production of ornamentals13. Protoplast isolation and fusion14. Agrobacterium-mediated transformation of plants14-1. Petunia or tobacco leaf disk14-2. Petunia shoot apex14-3. Tobacco leaf infiltration14-4. Arabidopsis floral dip transformation14-5. Dicot plant-based techniques and experiments14-6. Rice transformation techniques and experiments15. Genome edition and RNAi15-1. Tomato genome edition15.2. Lettuce RNAi

ISBN: 9780128211205

Page Count: 252

Retail Price :

9780123814678; 9780123743800; 9780124171046

Undergraduate and postgraduate students and researchers focusing on plant tissue culture/micro-propagation, horticulture, botany, biotechnology and plant physiology